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. Author manuscript; available in PMC: 2016 Nov 9.
Published in final edited form as: Cancer Cell. 2015 Nov 9;28(5):569–581. doi: 10.1016/j.ccell.2015.09.021

Figure 4. EGFR signaling via promoting glucose uptake upregulates SCAP and activates SREBP-1.

Figure 4

(A, B) Confocal microscopy images of SREBP-1 subcellular distribution (A), or western blot analysis of SREBP-1 cleavage (B) in U87/EGFR cells in response to EGF (50 ng/ml) in the presence or absence of glucose (5 mM) for 12 hr. Scale bars, 20µm. SREBP-1 nucleus intensity was quantified over 30 cells by using ImageJ. The results are shown as mean ± SEM (n = 30). Significance was determined by an unpaired student t test.

(C) Glucose uptake analysis of U87/EGFR cells in response to EGF (50 ng/ml) by using 14C-glucose isotope. The results are shown as mean ± SEM (n=3). Significance was determined by an unpaired student t test.

(D) Western blot analysis of cell membrane and nuclear extracts from U87/EGFR cells in response to EGF (50 ng/ml) stimulation in the presence or absence of glucose (5 mM) for 12 hr.

(E) Western blot analysis of U87/EGFR cells after knockdown of SCAP in comparison with shRNA control (shCtrl) in response to EGF (50 ng/ml) stimulation in the presence of glucose (5 mM) for 12 hr.

See also Figure S4.