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. Author manuscript; available in PMC: 2015 Nov 13.
Published in final edited form as: Biochem J. 2015 May 1;467(3):425–438. doi: 10.1042/BJ20131571

Figure 2. SILCS identified analogues of 2.3.2 inhibit Elk-1 phosphorylation.

Figure 2

(A)SILCS FragMaps overlaid on to the 2.3.2–ERK2 crystallographic structure. 3D probability distributions are shown for aliphatic (green) and aromatic (purple) functional groups. (B) The structures and change in LGFE scores (LGFE) for 2.3.2 (now referred to as SF-3-026) and analogues (SF-2-027, SF-2-029 and SF-2-030). (C and D) Lysates from HeLa cells pre-treated with 50 μM of SF-3-026 or analogues followed by stimulation with EGF for 10 min were immunoblotted for phosphorylated Elk-1 (pElk-1 Ser383) and RSK1 (pRSK1 Thr573) in (C) or ERK1/2 (pERK1/2) and MEK1/2 (pMEK1/2) in (D). Total Elk-1 and RSK1 or ERK2 and MEK1 are shown as protein loading controls in (C) or (D) respectively. Results are representative of three independent experiments.