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Nondegenerate clinical pyrograms and their confirmatory virtual pyrograms using the homebrew dispensation sequence. Shown are clinical pyrograms for A, V600E (c.1799T>A) and B, V600D (c.1799_1800delTGinsAT). Shown are pyrograms for C, V600K (c.1798_1799delGTinsAA) and D, V600R (c.1798_1799delGTinsAG). Figures E-H are the virtual pyrograms for these four mutations.
