Table 1. Expected Peak Changes for Pyrograms of BRAF Mutations.
| Mutationa | Homebrew Dispensation | Kit Dispensation | No. of Mutations | Activatingb | Supplemental Figurec |
|---|---|---|---|---|---|
| L597L (c.1791A>G) | 7,8d | Not detected | 3 | No | 1A |
| A598T (c.1792G>A) | 7,8d | Not detected | 1 | No | 1B |
| A598V (c.1793C>T) | 9,10 | 11,12e | 4 | Yes19 | 1C |
| A598_T599insV (c.1794_1795insGTT) | 11,13,14,18,20,21,22,23, 24, 25,26,27,29,30,31,32,34 | 11,12e | 6 | Yes20 | 1D |
| V600M (c.1798G>A) | 14,15 | 6,7,8 | 20 | No | 2A |
| V600R (c.1798_1799delGTinsAG) | 14,15,17,19,20,21,25,26 | 2,4,5,7,8e | 33 | Yes21,22 | 2B |
| T599_V600insT (c.1796_1797insTAC) | 14,15,20,21,22,23,24,25, 26, 27,29,30,31,32,34 | 4,5,7,8e | 3 | Yes23 | 1E |
| V600R (c.1797_1799delAGTinsGAG) | 14,17,18,20 | 2,4,5,8 | 1 | Probably22 | 2C |
| V600K (c.1798_1799delGTinsAA) | 14,17,19,20,21,25,26 | 4,5,7,8e | 183 | Yes21,22 | 2D |
| V600G (c.1799T>G) | 15,17,19,20,21,25,26 | 2,4,5,7,8e | 11 | No | 2E |
| V600E (c.1799T>A) | 17,18 | 4,5 | 14,200 | Yes1 | 2F |
| V600_K601delinsE (c.1799_1801delTGA) | 17,19,20,21,25,26d | Not sequenced | 12 | Yes22 | 2G |
| V600E (c.1799_1800delTGinsAA) | 17,19,20,21,25,26d | 1,4,5,7,8 | 29 | Probably1 | 2H |
| V600D (c.1799_1800delTGinsAT) | 17,20 | 2,6 | 14 | Yes1 | 2I |
| V600V (c.1800G>A) | 20,21d | 1,2 | 1 | No | 2J |
| K601E (c.1801A>G) | 20,21d | Not sequenced | 38 | Yes24 | 3A |
| K601N (c.1803A>C) | 21,22 | Not sequenced | 2 | Yes25 | 3B |
| K601K (c.1803A>G) | 21,23,24,25,26,28,30,31,32,33,34 | Not sequenced | 1 | No | 3C |
| K601N (c.1803A>T) | 21,23 | Not sequenced | 6 | No | 3D |
| K601I (c.1802A>T) | 21,24,25,26,27,30,31,32,33,34 | Not sequenced | 1 | No | 3E |
| V600_S605delinsEK (c.1799_1815delinsAAAAG) | 17,18,20,21,23,24,25,26,27,28,29,30,31,32,33,34 | Not sequenced | 1 | No | 4F |
| V600_S605delinsD (c.1799_1814delinsA) | 17,18,20,21,23,24,25,26,27,28, 29,30,31,32,33,34 | Not sequenced | 1 | No | 4C |
a
Codons 595 to 605 only.
b
Measured by ERK phosphorylation.
c
Refers to comprehensive figures (examples of many of these can be found elsewhere in the article).
d
Degenerate peaks when using the homebrew dispensation.
e
Degenerate peaks when using the kit-based dispensation.