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. Author manuscript; available in PMC: 2015 Nov 13.
Published in final edited form as: Clin Exp Allergy. 2014 Sep;44(9):1188–1199. doi: 10.1111/cea.12365

Figure 1.

Figure 1

Experimental protocol. Mice (6-weeks, 18-months) were infected with influenza A 3 days prior to antigen sensitization. Mice were sensitized intraperitoneally (i.p) with 100μg OVA absorbed with 2mg alum in 0.4ml PBS on days 0 and 7. Ten days after the last sensitization dosing, mice were anesthetized i.p. with ketamine and xylazine and challenged intratracheally (i.t.) for a total of four times with 100μg OVA on days 17, 24, 31 and 32. Control mice consisted of: age-matched antigen sensitized and challenged alone, virally infected alone (day -3), and naïve mice. Forty-eight hours after the final OVA challenge the mice were sacrificed.