Figure 2.
Gold nanoparticle association with bacterial cells is directly observable and depends on cell LPS content. (a) Cells isolated (sorted) from the total cell population after exposure to MPNH2-AuNPs. (b) Unsorted cells after exposure to MPNH2-AuNPs. In panels a and b the arrows point to AuNPs associated with the cells as confirmed by hyperspectral imaging. (c) Association of MPA- or MPNH2-AuNPs with Shewanella cells with varying LPS content (indicated by 8-amino-2-keto-3-deoxy-D-manno-octonate (8-aminoKdo) content of lyophilized cells) quantified by flow cytometry. Error bars (representing one standard deviation, n = 3) are smaller than the symbol in some cases.