Figure 3.
(a) Hydrodynamic diameters and (b) electrophoretic mobilities of unilamellar vesicles composed of POPC or POPC and the indicated mole percent of either smooth LPS (sLPS) or rough LPS (rLPS). Apparent ζ potentials derived from these electrophoretic mobility measurements are presented in Figure S7. All values were measured at a vesicle concentration of 10 μg·mL−1 in a 0.002 M HEPES (pH 7.4), 0.001 M NaCl solution. Error bars represent one standard deviation (n = 5). (c) Content of 2-keto-3-deoxy-D-manno-octonate (Kdo) in the POPC/LPS vesicles as a function of mass percent LPS addition (used to estimate mol % LPS in vesicles). Each smooth LPS molecule contains three Kdo residues while each rough LPS molecule contains two Kdo residues.45,46 Error bars represent one standard deviation (n = 3). Values labeled with different letters differ significantly from each other (p < 0.05).