Fig. 3. Selective loss of Gata2 expression in −77^−/− myeloid progenitors disrupts homeostasis.

(A) Representative flow cytometric analysis of E13.5 fetal livers for Lin⁻Sca1⁺Kit⁺ and Lin⁻Sca1⁻Kit⁺ cells, CMPs (Lin⁻CD34⁺FcγR^lowKit⁺Sca1⁻), GMPs (Lin⁻CD34⁺FcγR^highKit⁺Sca1⁻), and MEPs (Lin⁻CD34⁻FcγR^lowKit⁺Sca1⁻). (B) Percentages of Lin⁻Sca1⁺Kit⁺ and Lin⁻Sca1⁻Kit⁺ cells [top; −77^+/+ (n = 7), −77^+/− (n = 17), and −77^−/− (n = 6)] and CMPs, GMPs, and MEPs [bottom; −77^+/+ (n = 8), −77^+/− (n = 6), and −77^−/− (n = 9)]. (C) Quantitation of Gata2 mRNA levels in Lin⁻Sca1⁺Kit⁺ and Lin⁻Sca1⁻Kit⁺ cells [−77^+/+ (n = 5) and −77^−/− (n = 7)] and CMPs, GMPs, and MEPs [−77^+/+ (n = 4) and −77^−/− (n = 4)]. (D) Profiles for chromatin accessibility via the assay for transposase-accessible chromatin (ATAC) and histone H3K4 monomethylation mined from existing data (53). Graphs show means ± SEM; **P < 0.01, ***P < 0.001.