Fig. 6. The tuning of LN3 and LN4 results from the timing of AN1 and LN5 excitation and matches the phonotactic behavior.

(A) Response tuning of the coincidence detector LN3 (N = 10), the feature detector LN4 (N = 5), and the phonotactic behavior (N = 14) toward chirps with different pulse periods but constant sound energy (26). (B to G) Instantaneous spike rate of AN1 (red area; average n = 10) and changes in the membrane potential of LN5 (blue trace; signal average, n = 5) for chirps from the same paradigm as used in (A). Both interneurons were recorded subsequently in the same animal, independent of phonotaxis tests. See fig. S7 for m3ore details.