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(A) Applying glue to pin (B and C) attaching pin to eye (D) using microscissors to puncture and cut through sclera and retina (E) making two more cuts to have eye in three segments (F) applying glue to pin (G and H) attaching sclera to pin (I) removing lens and irrigating (J) setting pin in septum (K) setting septum in respirometry vial.
