Fig. 1. Lung transplant assessment assay.

(A) The lung assessment assay workflow. A biopsy is taken from a donated lung, and the cells are homogenized and lysed. The mRNA released from the cells is analyzed using a chip-based method that delivers a gene expression profile predictive of the outcome of a transplant within 20 min of the biopsy. (B) FraCS sensor chip (left) and SEM images of sensors after electrochemical deposition (right). (C) Assay readout [PNA probe (black) and resulting differential pulse voltammetry (DPV) signal (right panel, shown in dark blue), target mRNA (light blue) hybridization, and resulting DPV signal (right panel, shown in red)].