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. 2015 Aug 21;1(7):e1500263. doi: 10.1126/sciadv.1500263

Fig. 3. Proton relay stabilizing the imidic acid state of Asn92.

Fig. 3

(A) Difference maps calculated without H/D atoms around carbonyl oxygen atoms of Asn92 and the amide of Cys96. The 2Fobs − Fcalc (blue) and Fobs − Fcalc (red and green) maps of the x-ray analysis are shown at the 1.0σ and 3.0σ levels, respectively, and 2Fobs − Fcalc (purple) and Fobs − Fcalc (red and green) maps of the neutron analysis are shown at the 1.0σ and 2.0σ levels, respectively. (B) Difference maps calculated without H/D atoms around carbonyl oxygen atoms of Asn105 and amide of Asn105, His107, and Met109. The 2Fobs − Fcalc map of the x-ray analysis is shown at the 1.0σ level, and the Fobs − Fcalc map of the neutron analysis is shown at the 2.0σ level. (C) Proposed mechanism of formation of the imidic acid form of Asn92.