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. 2015 Nov 13;10(11):e0142939. doi: 10.1371/journal.pone.0142939

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© 2015 Zafir et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — A: Immunoblot showing decrease in total O-GlcNAcylated proteins 5 days post-differentiation (+Dex). B: Densitometry demonstrates a significant reduction in O-GlcNAc levels occur during dexamethasone-induced differentiation, relative to control, undifferentiated cells (-Dex). C: Differentiation-induced mRNA expression of early cardiac transcription factor Nkx2.5 is suppressed by maintenance of augmented O-GlcNAc levels during differentiation with the OGA inhibitor TMG. D: Early differentiation is also evidenced by enhanced cardiac actin expression, although unaffected by O-GlcNAcylation. Individual data points are plotted and the horizontal line represents the mean, n>/ = 4/group, *p < 0.05 vs–Dex/Control.