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. 2015 Nov 13;10(11):e0142939. doi: 10.1371/journal.pone.0142939

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© 2015 Zafir et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — A: Immunoblot (with mouse heart lysate as positive control), and B: densitometric analysis demonstrating a robust early (day 5) and significant increase in smooth muscle actin (SMA) expression during differentiation. SMA remained elevated for extended periods of differentiation (7 to 21 d). C: Immunoblot, and D: densitometry revealing that higher O-GlcNAc levels (maintained with TMG) induced a significantly higher level of SMA expression 5 days post-differentiation than Veh treated differentiating cells. Individual data points are plotted and the horizontal line represents the mean, n>/ = 3/group, *p < 0.05 vs day 0/Control; #: p < 0.05 vs Veh +5AZA.