Table 1. Up-regulated pneumococcal genes following adherence to human Met-5A PMC cells.
| Gene | Gene Symbol | t-Test p- value | Mean Fold- Expression Change | Gene Product |
|---|---|---|---|---|
| sptigr4-0136 | sp_0136 | 0.000156 | 3.6 | glycosyl transferase, family 2 |
| sptigr4-0137 | abc-np | 0.000447 | 4.7 | ABC transporter, ATP-binding protein |
| sptigr4-0138 | sp_0138 | 0.00055 | 3.6 | hypothetical protein |
| sptigr4-0139 | sp_0139 | 0.00604 | 4.0 | hypothetical protein |
| sptigr4-0140 | ugd | 0.00117 | 5.4 | UDP-glucose dehydrogenase |
| sptigr4-0144 | sp_0144 | 0.000407 | 4.6 | hypothetical protein |
| sptigr4-0145 | sp_0145 | 0.000135 | 6.2 | Hypothetical protein |
| sptigr4-0231 | adk | 0.00113 | 4.4 | Adenylate kinase |
| sptigr4-0366 | aliA | 0.00586 | 5.7 | oligopeptide ABC transporter, oligopeptide-binding protein AliA |
| sptigr4-0445 | ilvB | 0.00382 | 4.0 | acetolactate synthase catalytic subunit |
| sptigr4-0502 | glnA | 0.00268 | 3.8 | Glutamine synthetase, type I |
| sptigr4-0636 | abc-nbd | 0.000134 | 2.6 | ABC transporter, ATP-binding protein |
| sptigr4-0857 | sp_0857 | 0.000433 | 3.0 | ABC transporter substrate-binding protein |
| sptigr4-0858 | sp_0858 | 0.000227 | 2.4 | Hypothetical protein |
| sptigr4-0859 | sp_0859 | 0.00143 | 2.8 | Membrane protein |
| sptigr4-0862 | rpsA | 0.00393 | 6.4 | 30S ribosomal protein S1 |
| sptigr4-0957 | sp_0957 | 0.000838 | 2.9 | ABC transporter, ATP-binding protein |
| sptigr4-0961 | rplT | 0.00207 | 2.7 | 50S ribosomal protein L20 |
| sptigr4-0964 | pyrD | 0.000401 | 2.8 | Dihydroorotate dehydrogenase 1B |
| sptigr4-0965 | lytB | 0.00339 | 3.1 | endo-beta-N- acetylglucosaminidase |
| sptigr4-1241 | glnP | 0.000721 | 8.8 | Amino acid ABC transporter, amino acid-binding protein/permease protein |
| sptigr4-1242 | glnQ | 0.000302 | 10.0 | Amino acid ABC transporter, ATP-binding protein |
| sptigr4-1306 | gdhA | 0.0000353 | 8.7 | Glutamate dehydrogenase |
| sptigr4-1468 | pdx1 | 0.00138 | 6.4 | pyridoxine biosynthesis protein |
| sptigr4-1469 | nox | 0.000609 | 3.2 | NADH oxidase |
| sptigr4-1511 | atpH | 0.000547 | 3.3 | F0F1 ATP synthase subunit delta |
| sptigr4-1512 | atpF | 0.000216 | 2.6 | F0F1 ATP synthase subunit B |
| sptigr4-1513 | atpB | 0.000988 | 3.3 | F0F1 ATP synthase subunit A |
| sptigr4-1537 | sp_1537 | 0.000447 | 2.7 | hypothetical protein |
| sptigr4-1538 | sp_1538 | 0.00409 | 4.2 | Cof family protein/peptidyl-prolyl cis-trans isomerase, cyclophilin type |
| sptigr4-1597 | sp_1597 | 0.000216 | 3.0 | Hypothetical protein |
| sptigr4-1598 | pdxK | 0.000547 | 4.8 | Phosphomethylpyrimidine kinase |
| sptigr4-1599 | truA | 0.000447 | 5.8 | tRNA pseudouridine synthase A |
| sptigr4-1648 | psaB | 0.000216 | 4.0 | Manganese ABC transporter, ATP- binding protein |
| sptigr4-1715 | abc-nbd | 0.000407 | 4.0 | ABC transporter, ATP-binding protein |
| sptigr4-1855 | adhB | 0.00268 | 4.4 | alcohol dehydrogenase, zinc- containing |
| sptigr4-1856 | sp_1856 | 0.000227 | 3.6 | Transcriptional regulator, MerR family |
| sptigr4-1857 | czcD | 0.0000353 | 4.9 | Cation efflux system protein |
| sptigr4-1913 | sp_1913 | 0.000245 | 4.8 | Hypothetical protein, cspC-related protein |
| sptigr4-1914 | sp_1914 | 0.0000353 | 5.5 | Hypothetical protein |
| sptigr4-1992 | sp_1992 | 0.000447 | 2.6 | cell wall surface anchor family protein |
| spr6-1178 | spr1178 | 0.00116 | 3.9 | Hypothetical protein |
Differential gene expression was analyzed by microarray in pneumococci adherent to Met-5A cells at 2h post challenge. Genes demonstrating ≥ mean 2-fold changes in expression in all (n = 3) replicates with significance (t-Test p-value) are ordered by sp number.