. 2015 Nov 13;10(11):e0142272. doi: 10.1371/journal.pone.0142272

Table 6. Regression Analysis.

Simple regression–linear model: Independent variable, RNFL; dependent variables, NAA, N-acetyl-aspartate; Cho, choline; Cr, creatine; LL per BV, lesionload per Brain Volume, LL AR, lesion load along anterior right visual pathway; LL AL, lesion load along anterior left visual pathway; LL PR, load along posterior right visual pathway; LL PL, load along posterior left visual pathway; Evan’s Index; CHI; CMI; BCI; the maximum width of the 3^rd ventricle; the maximum width of the 4^th ventricle; MIF, the maximum width of the anterior interhemispheric fissure; MFSS, the maximum frontal subarachnoid space; MSF, the maximum width of the Sylvian fissure; DD, disease duration; EDSS, expanded disability severity scale. 1 ^st row: all right eyes (n = 28; with and without ON) of all included MS patient. 2 ^nd row: all left eyes (n = 28; with and without ON) of all included MS patient. 3 ^rd row: all right eyes of MS patients who never experienced an ON (neither on their right nor on their left eye; RRMS, n = 17, SPMS, n = 2; Table 1). 4 ^th row: all left eyes of MS patients who never experienced an ON (neither on their left nor on their right eye; RRMS, n = 17, SPMS, n = 2; Table 1). Patients are the same as in the 3^rd row. 5 ^th row: right eyes of 6 MS patients who experienced an ON on their right eyes (note, 4 out of 6 experienced ON on both eyes, 2 only on their right eyes; Table 1). 6 ^th row: left eyes of 6 MS patients who experienced an ON on their left eyes (note, 4 out of 7 experienced ON on both eyes, 3 only on their left eyes Table 1). For each analysis the correlation coefficient (corr. coeff.), R-squared (percent), the standard error of estimate (STE of Est.) and the p-value (analysis of variance, ANOVA) is given. Since the p-value in the ANOVA table is less than 0.01, there is a statistically significant relationship between the maximum width of the 4^th ventricle and the RNFL (for all patients’ right eyes, n = 28, 1^st row and for all patient’s left eyes, who never experienced ON, n = 17, 4^th row) at 99% confidence level. However, the low correlation coefficient indicates that there is only a weak relationship between the variables. R-squared statistic indicates that the simple/linear regression explains only 24.92% (1^st row) or 28.68% (4^th row) of the variability of the independent variable. In all other analyses presented here (and performed for the six OCT-Sectors, see material and methods or Table 5) no statistically significant correlation could be found (data not shown).

RNFL		NAA	Cho	Cr	LL, per	LL, AR	LL, AL	LL, PR	LL, PL	Evans	CHI	CMI	BCI	width	width	MFSS	MIF	DD	EDSS
					BV					ratio				of 3^rd	of 4^th
														ventricle	ventricle
right eye	Corr. Coeff.	0.041	0.059	0.136	0.151	0.010	0.241	0.029	0.084	0.067	0.123	0.236	-0.133	0.347	0.492	-0.187	0.061	-0.204	-0.279
n = 28	R-squared	0.17	0.35	1.87	2.30	0.01	5.79	0.088	0.712	0.450	1.527	5.616	1.785	12.1	24.29	3.511	0.371	4.17	7.28
	STE of Est.	2.41	0.36	1.12	0.58	168	123	566.2	451	0.05	0.04	0.07	0.02	2.63	1.463	0.79	0.87	7.11	1.71
	p-Value	0.83	0.76	0.49	0.44	0.96	0.21	0.88	0.67	0.73	0.53	0.22	0.49	0.07	0.007	0.34	0.75	0.30	0.16
left eye	Corr. Coeff.	0.115	0.076	0.150	0.240	0.201	0.313	0.203	0.257	-0.043	-0.076	0.090	-0.044	0.150	0.462	-0.092	-0.092	-0.312	-0.373
n = 28	R-squared	1.32	0.58	2.26	5.76	4.05	9.81	4.12	6.63	0.19	0.57	0.81	0.19	2.26	21.40	0.86	0.84	9.77	13.97
	STE of Est.	2.42	0.36	1.12	0.57	164.9	120.8	554.7	437.6	0.05	0.04	0.07	0.02	2.78	1.49	0.80	0.87	6.90	1.65
	p-Value	0.56	0.67	0.45	0.22	0.30	0.10	0.30	0.20	0.83	0.70	0.65	0.83	0.44	0.01	0.63	0.64	0.1	0.05
right eye,	Corr. Coeff.	0.151	0.05	0.31	-0.006	0.068	0.111	-0.043	-0.102	0.200	0.063	0.297	-0.188	0.329	0.387	-0.262	0.030	-0.248	-0.162
without ON	R-squared	2.30	0.23	9.76	0.01	0.47	1.23	0.19	1.05	4.01	0.40	8.86	3.52	10.88	14.92	6.89	0.09	6.13	2.63
n = 19	STE of Est.	2.44	0.42	1.21	0.54	119.2	125,0	525,3	334.8	0.05	0.04	0.07	0.02	2.86	1,40	0.75	0.93	7.39	1.29
	p-Value	0.54	0.84	0.20	0.97	0.78	0.61	0.86	0.68	0.41	0.80	0.21	0.44	0.17	0.10	0.28	0.90	0.31	0.51
left eye	Corr. Coeff.	0.257	0.078	0.335	0.002	0.060	0.082	-0.027	0.031	0.233	-0.073	0.244	-0.018	0.198	0.535	-0.250	-0.087	-0.387	-0.227
without ON	R-squared	6.60	0.60	11.26	0.001	0.36	0.69	0.07	0.10	5.45	0.54	5.97	0.03	3.93	28.68	6.26	0.75	14.94	5.16
n = 19	STE of Est.	2.39	0.43	1.19	0.53	120,59	125,38	525.60	336,47	0.05	0.04	0.07	0.02	2.97	1.28	0.75	0.94	7.04	1.27
	p-Value	0.29	0.75	0.16	0.10	0.81	0.74	0.91	0.90	0.34	0.76	0.31	0.94	0.42	0.02	0.30	0.72	0.10	0.35
right eye	Corr. Coeff.	0.407	0.126	0.077	-0.094	-0.387	0.302	-0.277	-0.293	0.333	0.221	-0.333	-0.333	0.107	0.686	-0.386	0.133	-0.687	-0.690
with ON	R-squared	16.60	1.60	0.59	0.87	15.01	9.12	7.69	8.57	11.12	4.87	11.12	11.12	1.15	46.92	14.89	1.78	47.21	47.65
n = 6	STE of Est.	2.06	2.32	0.63	0.41	241.75	119.20	433.31	349.74	0.04	0.04	0.04	0.04	1.15	1.47	0.86	0.92	3.41	2.04
	p-Value	0.42	0.81	0.88	0.86	0.45	0.56	0.59	0.57	0.51	0.67	0.52	0.52	0.84	0.13	0.45	0.80	0.13	0.13
left eye	Corr. Coeff.	-0.262	0.550	-0.515	0.354	0.134	0.627	0.361	0.286	-0.387	0.236	0.426	0.123	0.309	0.662	-0.129	0.041	-0.393	-0.504
with ON	R-squared	6.84	30.26	26.49	12.59	1.80	39.26	13.09	8.18	14.99	5.59	18.18	1.56	9.53	43.87	1.66	0.17	15.44	25.35
n = 7	STE of Est.	2.95	0.15	0.82	0.72	255.51	101.97	628.24	681.43	0.039	0.04	0.04	0.04	2.49	1.67	0.92	0.72	7.36	2.25
	p-Value	0.57	0.21	0.24	0.43	0.77	0.13	0.43	0.53	0.39	0.61	0.34	0.79	0.50	0.10	0.78	0.93	0.38	0.25