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. 2015 Nov 13;13(11):e1002288. doi: 10.1371/journal.pbio.1002288

Fig 4. The Δtgpts strain lacks all detectable PtdThr species and shows an evident increase in PtdSer.

Fig 4

Total lipids (107 tachyzoites) of the specified parasite strains were resolved by HPLC (not shown), and the eluted X1 fraction was subjected to MS analysis, as described in Fig 1. Exemplified spectra of the parental (top), Δtgpts (middle), and complemented (bottom) strains confirm the absence of a major (m/z 850.5, 40:5) and two minor (m/z 824.5, 38:4; m/z, 878.5, 42:5) PtdThr species in the Δtgpts strain. PtdSer-derived peaks are more intense in the Δtgpts strain, which is consistent with TLC (Fig 3D) and lipid phosphorus assays (S7 Fig). Unlike the parental strain, the Δtgpts mutant overexpressing TgPTS-HA lacks certain PtdSer species and shows additional minor PtdThr species, which is likely due to mutual regulation of PSS and PTS catalysis.