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. 2015 Nov 13;10(11):e0142901. doi: 10.1371/journal.pone.0142901

Fig 1. 3-NP induced increases in the mRNA and protein levels of HMGB1 and in the protein levels of p-JNK and caspase-3 in rat striatum in vivo.

Fig 1

The time-course of 3-NP–induced changes in HMGB1 expression, JNK phosphorylation, and caspase-3 cleavage was determined by isolating striatal tissue from rats that were killed 12 or 24 h after intrastriatal infusion of 3-NP (300 nmol) or vehicle control (CONT; isotonic saline solution, 1 μL). Striatal tissues were dissected for preparation of striatal extracts for immunoblotting. (A-B) 3-NP induced upregulation of the mRNA and protein levels of HMGB1. (C-D) 3-NP induced alterations in the levels of JNK phosphorylation and cleaved caspase-3. Densities of protein bands were analyzed with an image analyzer (Sigma Scan Pro 5) and normalized to the loading control (β-actin). Bars represent mean ± SE; n = 4 animals per group. Groups were compared by ANOVA followed by Dunnet’s post hoc test before data conversion. All panels, **p <0.01 vs control.