Table 1. Effect of donor cell types on in vivo development of cloned embryos.
Cell types | No. surrogates (embryos transferred) | No. pregnancy (%) | No. delivery (%) | No. piglets (mean ± SEM) | No. piglets alive (mean ± SEM) | Cloning efficiency (%) |
---|---|---|---|---|---|---|
L-PAFs | 11 (1930) | 8 (72.73 ± 14.60) | 7 (63.64 ± 15.13) | 27 (2.46 ± 0.87) | 18 (1.64 ± 0.60) | 0.99 ± 0.38 |
LW-PAFs | 46 (7905) | 29 (63.04 ± 7.14) | 26 (56.52 ± 7.40) | 113 (2.46 ± 0.43) | 74 (1.61 ± 0.29) | 0.97 ± 0.19 |
L-PAFs and LW-PAFs groups displayed no significant differences of in vivo development.
The rates of pregnancy and delivery and the average numbers of piglets born and alive were based on the number of surrogates.
Cloning efficiency was calculated by total number of live cloned piglets/total number of transferred cloned embryos.