FIG. 5.

RBBP4 KD perturbs CPC localization and activity during oocyte maturation. Full-grown, GV-intact oocytes were injected with the siRNA and morpholino cocktail, followed by IVM. Met I oocytes were fixed and immunostained with an anti-AURKC antibody (red in merge; A), anti-survivin antibody (red in merge; C), and anti-phospho-INCENP antibody (red in merge; D). DNA was detected with 4′,6-diamidino-2-phenylindole (blue in merge). In B, 100 ng/μl of Aurkc-Gfp (green in merge) cRNA was injected with the siRNA/morpholino mixture. The graphs to the right show the quantifications. The experiments were performed at least twice, and at least 15 oocytes were analyzed for each sample. Representative images are shown. The data are expressed as the mean ± SEM; Student t-test was used to analyze the data. *P < 0.05, **P < 0.01. Bars = 10 μm.