FIG. 6.

Overexpression of AURKC partially rescues the RBBP4 KD phenotypes. Full-grown oocytes were injected with the indicated materials, followed by IVM. A) After 18 h, meiotic progression was evaluated, followed by fixation and staining of spindles with an anti-α-tubulin antibody (yellow in merge), phospho-INCENP (red in merge), and DNA with 4′,6-diamidino-2-phenylindole (blue in merge). Representative confocal microscopy images are shown. B–D) Quantification of the percentage of oocytes in A that extruded a polar body (PBE; B) had misaligned chromosomes (C), contained abnormal spindles (D), and displayed multipolar spindles (E). The experiments were performed three times, and at least 15 oocytes were analyzed for each sample. The data are expressed as the mean ± SEM, and one-way ANOVA was used to analyze the data. *P < 0.05, **P < 0.01. Bar = 10 μm.