FIG. 7.

Knockdown of RBBP4 and RBBP7 perturbs meiotic progression and chromosome segregation. Full-grown, GV-intact oocytes were injected with combination of siRNAs and morpholinos, followed by IVM for 18 h. A) Representative confocal microscopy images of the Met II spindle detected with anti-β-tubulin antibody (green) and DNA with TOPRO-3 (red) are shown. The percentage of misaligned chromosomes and abnormal spindle formation was assessed from these images. B) Met II eggs were examined for aneuploidy using an in situ chromosome spread. Kinetochores were stained with CREST anti-serum, and DNA was detected by TOPRO-3 staining. C) First PBE was assessed to evaluate meiotic progression. The experiments were carried out at least three times, and at least 20 oocytes were examined for each sample. The data are expressed as the mean ± SEM, and Student t-test was used to analyze the data. *P < 0.05, ***P < 0.001. Bar = 10 μm.