FIG. 1.

LacZ knockin and floxed alleles of Rxfp1. A) Schematic representation of Rfxp1^LacZ, Rxfp1^fl, and Rxfp1^Δ alleles. Exons are shown as black boxes and marked by a number underneath. SA is a splicing acceptor; IRES is an internal ribosome entry site; b-Gal is a β-galactosidase gene; pA is a poly(A) signal; beta-actin::Neo is neomycine-resistant gene driven by β-actin promoter; FLP is flp recombinase; CRE is cre recombinase. There are three LoxP sites for Cre recombinase on the 5′ and 3′ ends of the neo cassette and within intron 4. Rxfp1^fl allele is produced by flp-induced recombination, and the deleted allele without exon 4 is produced by cre-induced recombination as shown. The position of the primers used for genotyping is shown with arrows. B) Detection of Rxfp1^fl floxed allele with primers FP/RP1. The floxed allele produced a 298-bp band and the wild-type allele produced a 124-bp band. C) Detection of Rxfp1^Δ deleted allele with FP/RP2 primers. The floxed allele produced a 1100-bp band and the deleted allele produced a 189-bp band. The wild-type allele is not detectable with this primer pair.