Skip to main content
. 2015 Nov 13;10(11):e0142523. doi: 10.1371/journal.pone.0142523

Fig 2. Innate immune activation by Mycoplasma hyorhinis-infected B16 cells is fully dependent on MyD88 signaling but only partially on TLR2.

Fig 2

(A) Bone marrow cells or (B) differentiated GM-CSF DCs from wild-type, MyD88- or TRIF-deficient mice were cultured in the presence of diluted supernatant from Mycoplasma hyorhinis-infected B16 cells for 18 h. IL-6 levels in the immune cell culture supernatant were analyzed by ELISA. (C) GM-CSF DCs from wild-type and TLR2-deficient mice were cultured as in (A) in the presence of diluted supernatant from Mycoplasma hyorhinis-infected B16 cells and IL-6 levels in the DC culture supernatant were analyzed by ELISA. (D) GM-CSF DCs from wild-type mice were cultured as in (A). In some conditions, the diluted B16 culture supernatant was decontaminated by filtration (red bars), UV irradiation (blue bars) or both (purple bars) before it was added to the DC culture. IL-6 levels in the DC culture supernatant were analyzed by ELISA after 18 h. Data give the mean + S.E.M. of triplicate samples and are representative of at least two independent experiments. Asterisks indicate statistically significant differences to the appropriate wild-type control (in A-C) or to the appropriate non-decontaminated conditions (in D).