Fig 3. Effect of A) divalent cations and B) specificity of the phosphoryl donor of AaMVK.

Kinase activities were measured by the enzyme coupled spectrophotometric assay. A) Metal ion dependence was measured in the presence of ATP (250 μM) and MA (200 μM). Each value represents the means ± S.E. of three replicate assays. B) The specificity of phosphoryl donor were measured in the presence of 10 mM MgCl₂. Relative activity is defined as a percentage of the highest value recorded (ATP). Each value represents the means ± S.E. of three replicate assays (** P ≤ 0.05).