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. 2015 Nov 13;10(11):e0143107. doi: 10.1371/journal.pone.0143107

Fig 5. Inhibition of AaMVK activity by FPP in thorax extracts.

Fig 5

A) The activity of AaMVK in the absence and presence of FPP was measured by the enzyme-coupled spectrophotometric assay. B) The endogenous levels of IPP derived from the activity of AaMVK in thoraces extracts were measured by RP-HPLC. Optimal reaction conditions were used: ATP (250 μM) and MA (200 μM). Controls did not include substrate or cofactor. Data are expressed as percentage of the highest value recorded. Bars represent the means ± SE of three replicates of extracts from groups of 3 thoraces. Different letters above the columns indicate significant differences among treatments (one way ANOVA p < 0.05, with Tukey’s test of multiple comparisons).