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. 2015 Nov 13;10(11):e0142161. doi: 10.1371/journal.pone.0142161

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© 2015 Hurtado de Mendoza et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Histograms showing LFG staining in blue compared to IgG control in red. The left panel is gated on CD8 T cells and the right panel on CD4 T cells. (B) FACS plots representing LFG and CD44 expression before (red) or after TCR stimulation by adding anti-CD3/CD28 (blue) in CD8 (left panel) and CD4 (right panel) T cells. The CD44^hi/lo gate was created according to the unstimulated condition. The bottom panel represents the values of LFG mean fluorescence intensity for CD8 or CD4 T cells in the no stimulation versus the CD3/CD28 condition. (C) Histograms representing LFG expression in CD4 (blue), CD8 (green) and non-T cells (red) before and after CD3/CD28 stimulation. (n = 4 mice, *p ≤ 0.05, **p ≤ 0.01, ± = SEM).