Fig 2. CREBA133 mice phenotype (A), and hematoxylin and eosin (B, D) and Masson’s trichrome (C, E) stained cross-sections through the ventricles of wildtype (B), early HF (C), acute HF (D), and chronic HF (E) mice.
An acute HF mouse (A, right) is next to a wildtype littermate (A, left); note the significant anasarca in acute HF. In histological section, chambers are dilated and thinner with progression of HF. Insets in C show margins of an intracardiac thrombus in two different sectional planes. In the bottom inset panel, a fibrous band (blue) attaches the thrombus (150 μm x 100 μm) to the endocardium. Endocardial thrombi are present in early and acute HF mice with limited incidence in chronic HF. In E, post-surgical hemorrhage is seen within ventricles (orange/brown-staining with trichrome), without endocardial attachment. Scale bar in E is 1 mm for all panels (0.5 mm for insets).