Fig 4. Thrombin (FIIa; A) and APC (B) generation, and TM (C, D), EPCR (E, F), and Protein S (G, H) expression, on the endocardial surface of wildtype, all HF (HF), early HF, acute HF, and chronic HF mice.

Thrombin generation is increased in acute HF compared to wildtype mice (A). APC generation is decreased in all HF mice compared to wildtype mice (B). TM (C) and EPCR (E) mRNA expression is attenuated in all HF mice groups compared to wildtype mice. Protein S (G) transcripts are also decreased in early and chronic HF mice. Protein expression of TM (D) and Protein S (H) is decreased in chronic HF compared to wildtype mice. EPCR (F) protein expression is attenuated in early and acute HF mice relative to wildtype and chronic HF mice. *p<0.05, **p<0.01, ***p<0.005.