Fig 5. Tail clot assay (A) and platelet aggregometry (B-D) in wildtype, all HF (HF), early HF, acute HF, and chronic HF mice.

The bleeding index, reflecting time to clot and total blood loss, is decreased in early and acute HF mice compared to wildtype and chronic HF mice (A; p<0.005). Platelet aggregation with ADP is similar across all groups (B). In contrast, platelet agglutination with ristocetin is attenuated overall in HF mice (p<0.05; representative trace in C, data in D), particularly in acute HF mice.