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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Methods Mol Biol. 2016;1369:113–123. doi: 10.1007/978-1-4939-3145-3_9

Figure 1. Schematic of the procedure used for sample preparation for electron microscopy analysis.

Figure 1

After cell growth, the cells are harvested by vacuum filtration and vitrified by high pressure freezing. Two strategies can then be employed. Either the vitrified cells are cryo-sectioned right before observation in the scope (or storage) or the cells are freeze substituted, embedded in resin at room temperature and sectioned.