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. 2012 Apr 25;32(17):5804–5809. doi: 10.1523/JNEUROSCI.4849-11.2012

Figure 2.

Figure 2.

Pharmacological and transgenic confirmation that the 5-HT5A receptor mediates the unidentified 5-HT current. A, Voltage-clamp traces showing the unidentified 5-HT current in the presence of WAY-100635 (WAY) and ketanserin (Ket) (1.) can be re-elicited upon repeat application of 5-HT following sufficient washout (5 min) (2.). B, Under these conditions, the unidentified 5-HT outward current was not elicited by the 5-HT1A agonist, 8-OH-DPAT (paired t test; rat, p = 0.0007; mice, p < 0.0001), but was elicited by the mixed 5-HT receptor agonist, 5-CT (paired t test; rat, p = 0.7; mice, p = 0.4). C, In 5-HT5A+/+ mice, a substantial proportion of layer V neurons display an unidentified 5-HT current (squares). In sibling 5-HT5A−/− mice, however, layer V neurons do not display this current (triangles; Fisher's exact test, p = 0.0003). Inset, PCR products derived from 5-HT5A wild-type (+/+), heterozygous knock-out (+/−), and homozygous knock-out (−/−) mice. Lane M corresponds to a 100 bp DNA ladder with the 500 bp marker labeled.