The effect of exogenously applied thrombin receptor activating peptide (TRAP) on cortical neuron culture. A – D. Photomicrographs of 16 – 20 day old cultured primary rat cortical neurons stained for the neuron-specific cytoskeletal protein, MAP-2, after 24 hours of exposure to varied concentrations of TRAP (0, 10, 100, 200 nM). Yellow scale bars approximate 40 microns in length. E and F are graphical summaries of total measured neurite length and total number of neurites counted. All morphometric analysis was performed by experimenters blinded to experimental condition. Results are mean ± SD (n = 6, 6, 5, 5). P-value is greater than 0.98 for group comparison of total length and greater than 0.84 for group comparison of total number of neurites by one-way repeated measures analysis of variance (ANOVA).