Table 3.
Morphology, Immunophenotype, TCR clonality and Mutation analysis
| Final No |
Diagnosis | Size cells | Necrosis | Perivascular cuffing |
Meningeal spread |
CD2 | CD3 | CD4 | CD8 | CD5 | CD7 | CD56 | BF1 | TCRγ | TIA-1 | GrB | Perf | CD30 | ALK1 | EBV | KI67 | TRG PCR |
Ion Torrent TCLP39 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | PTCL, NOS | small-medium | pos | pos | neg | na | pos | pos | neg | pos | na | equiv | na | na | na | na | na | na | na | neg (LPM1) | na | pos | na |
| 2 | PTCL, NOS | medium | pos | pos | pos | pos | pos | neg | pos | pos f | neg | neg | pos | na | pos | pos | na | neg | na | neg (LPM1) | na | pos | na |
| 3 | PTCL, NOS | small | neg | neg | neg | pos | pos | pos | neg | pos | pos f | neg | pos | na | pos | neg | neg | neg | na | neg | mod | susp | DNMT3A, c.2207G>T, p.Arg736Leu |
| 4 | PTCL, NOS | small-medium | pos f | pos | neg | pos f | pos | neg | pos | pos | pos | neg | pos | na | pos | neg | na | neg | neg | neg | high | pos | WT |
| 5 | PTCL, NOS | medium | pos | neg | neg | pos | pos | mix | mix | pos f | pos f | neg | pos | na | pos | neg | neg | neg | neg | rare | mod | rest | WT |
| 6 | PTCL, NOS | large | neg | neg | neg | pos | pos | pos | neg | pos f | pos f | not interp | pos | na | neg | neg | neg | neg | neg | neg | high | no amp | na |
| 7 | PTCL, NOS | medium-large | pos | pos | neg | pos | pos | pos | neg | pos | pos f | neg | pos | neg | pos | pos | pos f | neg | neg | neg | mod | pos | WT |
| 8 | PTCL, NOS | medium-large | pos | pos | neg | pos | pos | pos | neg | pos | pos f | neg | na | na | na | na | na | neg | na | neg | mod | pos | na |
| 9 | PTCL, NOS | small-medium | neg | neg | neg | neg | pos | neg | pos | neg | pos | neg | pos | neg | pos | pos | pos | neg | na | na | high | pos | KRAS, c.34G>A, p.Gly12Ser; STAT5B, c.1924A>C, p.Asn642His; JAK3, c.1533G>T, p.Met511Ile |
| 10 | PTCL, NOS | small-medium | pos | pos | pos | pos | pos | neg | pos | pos f | pos | neg | pos | neg | pos | pos | pos | neg | neg | neg | high | pos | WT |
| 11 | PTCL, NOS | medium-large | pos | pos | pos | pos | pos | neg | pos | pos f | na | na | pos | neg | pos | pos | na | neg | neg | neg | na | pos | WT |
| 12 | PTCL, NOS | small | pos | pos | pos | na | pos | neg | pos | pos f | na | neg | neg | na | pos | na | na | na | na | rare | high | pos | na |
| 13 | PTCL, NOS γδ | small-medium | neg | pos | neg | pos f | pos | neg | pos | pos | pos | na | neg | pos | pos | pos | na | neg | na | neg | mod | pos | WT |
| 14 | PTCL, NOS γδ | small-medium | neg | pos | neg | na | pos | neg | pos | neg | na | na | neg | pos | na | na | na | na | na | na | mod | pos | TET2, c.4034A>C, p.Tyr1345Ser |
| 15 | PTCL, NOS TCR silent | medium | neg | neg | neg | pos | pos | neg | pos | neg | pos | neg | neg | neg | pos | pos | na | neg | na | neg | high | pos | GNB1,c.232A>G, p.Lys78Glu; STAT3, c.1981G>C, p.Asp661His |
| 16 | ALCL, ALK pos | medium-large; “Hallmark” cells | pos | pos | pos* | neg | neg | neg | neg | neg | neg | neg | US | na | pos | pos | pos | pos | pos c | neg | high | pos | WT |
| 17 | ALCL, ALK neg | large, “hallmark” cells | neg | neg | neg | pos | pos | neg | pos w | pos | pos f | not interp | pos | neg | pos f | pos | na | pos | neg | na | mod | no amp | na |
| 18 | ALCL, ALK neg | large, “hallmark” cells | pos | neg | neg | na | pos w | na | na | neg | na | neg | na | na | neg | na | na | pos | neg | neg | high | pos | na |
Abbreviations: pos- positive; neg- negative; f - focal, w - weak, mix – mixed, both CD4 and CD8 positive cells present, na- not available; not interp - not interpretable, βF1- T-cell receptor beta F1; TCRγ- T-cell receptor gamma; GrB- Granzyme B; mod- moderate; TRG – T-cell receptor gene rearrangement; no amp- no amplification products; rest - restricted; susp - suspicious; EBV- Epstein-Barr virus; LMP1- EBV latent membrane protein, US – unsatisfactory.