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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Metab Brain Dis. 2015 Aug 6;30(6):1515–1529. doi: 10.1007/s11011-015-9714-9

Fig. 1. Yield and purity of primary microglia cultures isolated from adult naïve mice.

Fig. 1

a. Summarizes the harvest yields on the day of MG isolation (mixed glial cells) and then after 21 days in vitro; with mixed glial cells cultured with 5 ng/ml GM-CSF through all the days. b. The purity of the MG was determined by staining for expression of CD11b and CD45. Values are given as mean ± S.E.M. Data presented are representative of n = 8 separate MG isolations and primary culture set-ups for naïve male WT mice. Statistical analysis was performed using Student’s t-test. Significant differences between sample means are indicated as ***P≤0.001 as compared to the values from day of MG isolation