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(a) Schematic of the microfluidic co-culture. (b) FITC-dextran transport across bEnd.3 cells on PTFE (n = 3, error bars are S.D.) and PE (n = 2) membrane-integrated microfluidic devices. (c) Phalloidin (green)-stained C8D1A astrocytes in collagen hydrogel in co-culture with bEnd.3 at day 14. Blue: Hoechst 33 342 counterstain. Scale bar: 50 μm.
