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. 2015 Nov 6;10:1825–1837. doi: 10.2147/CIA.S93819

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© 2015 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Chromatographic fingerprint analysis and cell viability assay for the herbal formula B401.

Notes: HPLC fingerprint of the herbal formula B401. Characteristic peaks of B401, ie, (Aa) ginsenosides Rb1 (from Panax ginseng) and oleanolic acid (from Ligustri fructus), (Ab) formononetin (from Astragalus membranaceus), (Ac) 5-HMF (from Rehmannia glutinosa), (Ad) ferulic acid (from L. fructus), (Ae) wedelolactone (from Eclipta prostrata), were identified and marked at the corresponding peaks in the fingerprint. (B) Cell viabilities of RA-induced SH-SY5Y cells in the absence (Ctrl) or presence of the B401 at indicated doses. The herbal formula B401 has no cytotoxicity in SH-SY5Y cells under treatment at a dose of less than 80 mg/mL.

Abbreviations: AU, arbitrary unit; IC₅₀, half maximal inhibitory concentration; 5-HMF, 5-hydroxymethylfurfural; RA, retinoic acid; Ctrl, control group.