Figure 7.

Expressions of ER-stress-related calpain in the brain tissue of the R6/2 (HD) mice were reduced under oral B401 treatment.

Notes: (A) IHC staining shows that the expressions of calpain in the striatum, hippocampus, and medulla of the 10-week-old R6/2 mice given the oral B401 treatment were obviously weaker than those given the sham treatment but had shown no difference in their WT. Scale bars: 30 µm. Western blotting analysis shows the following: (Ba) expression levels of calpain in whole brain tissue of the 10-week-old R6/2 mice given both oral B401 and sham treatments, and their WT and (Bb) quantified brain calpain levels of the 10-week-old R6/2 mice were significantly higher than their WT and significantly lower under oral B401 treatment. The number of R6/2 mice under oral B401 and sham treatments, and their WT was six for each group. Values are mean ± SEM (**P<0.01, two-way ANOVA followed by a Student–Newman–Keuls multiple comparison posttest).

Abbreviations: ER, endoplasmic reticulum; HD, Huntington’s disease; IHC, immunohistochemistry; WT, wild-type littermate; ANOVA, analysis of variance; SEM, standard error of the mean.