Fig. 4.

The MET protein was downregulated by siRNA in AGS and SGC-7901 cells. a si-MET-1/2/3 mimics and non-specific siRNA mimics were transfected into AGS cells. MET and survivin protein expression levels were determined after 72 h. b The data showed that MET protein expression levels were suppressed by 96.74, 95.86, and 94.95 %, respectively, for si-MET-1, 2, and 3. c Relative expression of survivin protein in AGS cells. d MET protein expression in SGC-7901 cells. e The protein expression was inhibited by 46.9, 61.2, and 54.8 %, respectively, after transfection with si-MET-1, 2, and 3. f Relative of survivin protein in SGC-7901 cells. The densities of the bands of MET and survivin expression were then quantified by Odyssey software 3.0 (LI-COR Biosciences). The data were then analyzed using Prism 5.0 software and expressed as mean ± SEM