Figure 1.
Nucleation and Growth of Snf7 Patches on Supported Membranes
Lipid composition is DOPC 60% / DOPS 40%+ Rhodamine PE 0.1%.
(A) Time-lapse images of Snf7-Alexa488 patches growth (green) at [Snf7] = 400 nM on supported membrane (gray).
(B) Time-lapse images (every 10 min) of a single Snf7-Alexa488 patch (green) growing at [Snf7] = 200 nM.
(C) Patch nucleation rate as a function of [Snf7].
(D) Successive (from bright to dark green, every 10 min) Snf7 patch fluorescence profiles (circularly averaged) at [Snf7] = 200 nM.
(E) Snf7 patch edge fluorescence profile (average of 3 patches) as a function of [Snf7] (data for [Snf7] < 200 nM were obtained by first nucleating the patches at 350 nM for 5 min, and then [Snf7] was reduced to the desired value).
(F) Exchange of bulk Snf7-Alexa488 (green) with Snf7-Atto647N (red) at 200 nM. Inset: kymograph of the region selected (yellow box). The green line is the switch between green and red Snf7.
(G) Equatorial kymograph of the patch shown in B.
(H) Patch radial growth speed as a function of [Snf7]. The slope of the linear fit (gray line) is 760 nm.min−1.μM−1.
See also Figure S1.