Figure 6.

Snf7 Lateral Pressure and Expansion Induced Membrane Deformations

(A) Confocal sections of Snf7 coated vesicles displaying stable holes. Fluorescence is more intense at the rim of the pore.

(B) EM thin section image of a Snf7 coated vesicle with a stable pore. Note the curling of the membrane rim. Several other examples of membrane curling are shown in lower panels.

(C) Sketch of the expected curvature generated by expansion of compressed Snf7 spirals.

(D) Sketch of the pore opening and curling of Snf7 coated vesicle. Bottom images show the expected section of a stable pore in the GUV and a zoom on the membrane curled region.