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. 2015 Nov 5;163(4):866–879. doi: 10.1016/j.cell.2015.10.017

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Figure S6 — Microscope Calibration with Labeled DNA Origamis, Related to Experimental Procedures

DNA origamis are labeled with Atto-647N (GATTAquant Brightness 9R: 9 Atto-647N molecules and GATTAquant Brightness 18R: 18 Atto-647N molecules). (A) Fluorescence histograms of diffraction limited fluorescent spots (see B) of GATTA-Brightness 9R (blue, n = 985) and GATTA-Brightness 18R (orange, n = 892). (B) Typical field of view of adhered DNA origamis appearing as diffraction limited spots. (C) and (D) Average fluorescence of DNA origamis spots (9R, blue and 18R, orange), varying linearly with laser power (expressed in percentage of maximal output) in (C) and with exposure time in (D). Error bars are SD of each fluorescence distribution measured.