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. 2014 Dec 5;77:14–17. doi: 10.1016/j.curtheres.2014.11.001

Figure 1.

Figure 1

The Escherichia coli DNA gyrase cleavage assay. DNA gyrase was incubated with supercoiled pBR322 in the presence of 0.1 µg/ML ciprofloxacin (CFX) and/or 1 of the phosphodiesterase inhibitors (100 µM). Bands were separated using 1% agarose and visualized using ethidium bromide. Phosphodiesterase inhibitors (100 µM) did not affect antigyrase activity of 0.1 µg/mL CFX.