Fig. 2.

BMP4-treated hPSCs express a new transient long DMD transcript. a qRT-PCR of DMD transcripts using primers specific to exons 2–3, 20–21, 45–46, and 64–65. Black bars represent mean ± SD from nine hPSCs at days 0 through 4 after BMP4 treatment. A muscle biopsy from a healthy individual was used as a control (white bar). Gene expression was normalized to mean of GAPDH and UBC and plotted (log10 scale) relative to mean expression of all hPSCs at D0. b qRT-PCR of DMD transcripts using primers specific to exons 2–3, Dp427c, and the new DMD transcript. On the left side, data represent the mean ± SD from nine hPSCs from days 0 through 4 after BMP4 treatment. On the right are data from expression in cultured normal myoblasts and myotubes, as well as in muscle biopsied from a healthy individual. Gene expression was normalized to mean of GAPDH and UBC and plotted (log10 scale) relative to mean expression of all hPSCs at D0. *p < 0.05, **p < 0.01, ***p < 0.001, and ns for not statistically significant