Table 1.
Generation of mature hematopoietic cells from pluripotent stem cell sources.
| Cell type | PSC source | Embryoid bodies | FCS | Feeder cells | Cytokines | Reference | |
|---|---|---|---|---|---|---|---|
| Myeloid cells | Erythrocytes | hESC (H1) | Yes | Yes | No | bFGF, VEGF, EPO, SCF, nFlt3-L, IL-3, IL-6, G-CSF, TPO | Chang et al (2006) |
| hESC (H1) | No | Yes | S17 and FH-B-hTERT (mFL stroma cells) | Clonogenic assay: SCF, GM-CSF, IL-3, EPO | Qiu et al (2005) | ||
| hESC | No | Yes | mFL stroma cells | Clonogenic assay: SCF, IL-3, IL-6, TPO, G-CSF, EPO | Ma et al (2008) | ||
| hESC (H1), hiPSC | No | Yes | OP9, MS5 | TPO, IL-3, IL-6, Flt3-L, SCF, EPO | Dias et al (2011) | ||
| hESC (H1) | No | Yes | FH-B-hTERT, MS5 | IL-3, BMP4, Flt3-L, SCF, EPO, IGF-1 | Olivier et al (2006) | ||
| Megakaryocytes/platelets | hESC (HES3, Envy, MEL1) | Yes | No | No | BMP4, VEGF, bFGF, SCF, TPO, IL-3 | Pick et al (2013) | |
| hESC (WA01) | Yes | Yes | OP9 | BMP4, VEGF, IL-3, Flt3-L, TPO, SCF, EPO | Vanhee et al (2015) | ||
| hESC (MA09, NED07), hiPSC | No | No | No | BMP4, VEGF, bFGF, TPO, SCF, Flt3-L, IL-3, IL-6, IL-9 | Feng et al (2014) | ||
| hESC (HuES3, MA01, MA09) | No | No | OP9, C3H | BMP4, VEGF, IL-6, IL-9, IL-11, bFGF, TPO, SCF | Lu et al (2011) | ||
| Granulocytes | hESC (KhES-3) | Yes | Yes | No | IGF-II, VEGF, SCF, Flt3-L, TPO, G-CSF | Saeki et al (2009) | |
| hESC (KhES-3) | Yes | Yes | OP9 | BMP4, SCF, Flt3-L, IL-6, TPO, G-CSF | Yokoyama et al (2009) | ||
| hESC (KhES-1, 3), hiPSC | Yes | No | No | BMP4, VEGF, SCF, TPO, Flt3-L, IL-3, G-CSF | Niwa et al (2011) | ||
| hiPSC | Yes | No | No | IL-3, G-CSF or GM-CSF | Lachmann et al (2015) | ||
| hESC (H1, H9), hiPSC | No | Yes | OP9 | GM-CSF, G-CSF, IL-3, IL-5 | Choi et al (2009) | ||
| MΦ | hESC (HUES-2, KCL001, 002) | Yes | No | No | IL-3, M-CSF | Karlsson et al (2008), van Wilgenburg et al (2013) | |
| hESC (H9), hiPSC | Yes | No | No | IL-3, M-CSF, or GM-CSF | Lachmann et al (2015) | ||
| hESC (H1, H9), hiPSC | No | Yes | OP9 | GM-CSF, M-CSF, IL-1b | Choi et al (2009) | ||
| DCs | hESC (H9) | Yes | hAS | No | SCF, Flt3-L, GM-CSF, IL-3, TPO, IL-4, TNF-α | Su et al (2008) | |
| hIPSC | No | Yes | OP9 | GM-CSF, M-SCF, IL-4, TNF-α | Senju et al (2011) | ||
| hESC (H1, H9), hiPSC | No | Yes | OP9 | GM-CSF, IL-4, TNF-α | Choi et al (2009) | ||
| Lymphoid cells | NK cells | hESSC (H1, HES-2), hiPSC | Yes | Yes | OP9-DL4 | BMP4, bFGF, Activin A, VEGF, IGF-1, IL-6, IL-11, SCF, IL-3, EPO, TPO, IL-13, Flt3-L, IL-15 | Sturgeon et al (2014) |
| hESC (H9), hiPSC | Yes | hAS | OP9-DL1 | BMP-4, VEGF, SCF, IL-3, Il-6, TPO, EPO, IL-7, Flt3-L, IL-15 | Ferrell et al (2015) | ||
| hESC (H9) | No | Yes | S17, AFT024 (mFL cells) | IL-15, IL-3, IL-7, SCF, Flt3-L | Woll et al (2005) | ||
| T cells | hESC (H1) | Yes | Yes | OP9-DL4 | BMP-4, bFGF, Activin A, VEGF, IL-6, IGF-1, IL-11, SCF, EPO, TPO, Flt3-L, IL-7, IL-15 | Kennedy et al (2012) | |
| hESSC (H1, HES-2), hiPSC | Yes | Yes | OP9-DL4 | BMP4, bFGF, Activin A, VEGF, IGF-1, IL-6, IL-11, SCF, IL-3, EPO, TPO, IL-3, Flt3-L, IL-7 | Sturgeon et al (2014) | ||
| hESC (H1, H9), hiPSC | No | Yes | OP9-DL1, OP9-DL4 | BMP-4, bFGF, VEGF, TPO, SCF, IL-6, IL-3, IL-7, Flt3-L | Uenishi et al (2014) | ||
| hESC (H1) | No | Yes | OP9, OP9-DL1 | Flt3-L, IL-7, SCF | Timmermans et al (2009) | ||
| B cells | hESC (H1, H9, ES03), | Yes | Yes | OP9 | BMP4, VEGF, FGF1, bFGF, SCF, Flt3-L, TPO, GM-CSF, IL-2, IL-4, IL-15, G-CSF, IL-3, IL-6, IL-7 | Zambidis et al (2008) | |
| hIPSC | No | Yes | OP9, MS5 | IL-7, IL-3, SCF, Flt3-L | French et al (2015) |
PSC, pluripotent stem cells; hESC, human embryonic stem cells; hiPS, human-induced pluripotent stem cells; bFGF, basic fibroblast growth factor; VEGF, vascular endothelial growth factor; EPO, erythropoeitin; SCF, stem cell factor; Flt3-L, FMS-like tyrosine kinase 3 ligand; IL-3, interleukin-3; IL-6, interleukin-6; G-CSF, granulocyte colony-stimulating factor; TPO, thrombopoietin; FL, fetal liver; GM-CSF, granulocyte–macrophage colony-stimulating factor; BMP4, bone morphogenic protein 4; IGF-1, insulin-like growth factor 1; IL-9, interleukin-9; IL-11, interleukin-11; IGF-II, insulin like-growth factor II; M-CSF, macrophage colony-stimulating factor; IL-1b, interleukin-1b; IL-4, interleukin-4; TNF-α, tumor necrosis factor-alpha; IL-7, interleukin-7; FGF1, fibroblast growth factor 1; IL-15, interleukin-15; hAS, human antibody serum; MΦ, macrophages; DCs, dendritic cells; NK, natural killer cells.