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. 2015 Sep 28;7(11):1426–1449. doi: 10.15252/emmm.201404976

Figure EV4. Effect of LATS1/2 on the growth of cancerous cervical cells.

Figure EV4

  • A Western blot analysis showed that LATS1/2 siRNAs successfully knocked down LATS1/2 and activated YAP in ME180 cells.
  • B Knockdown of LATS1/2 promoted proliferation of ME180 cells. Each point represents the mean ± SEM of five independent experimental results. **P < 0.01 (P = 0.0020).
  • C Representative images showing the anchorage-independent growth of ME180 with or without LATS1/2 knockdown (n = 5). Scale bar: 1 mm.
  • D, E The concentrations of AREG in 2D (D) or 3D hanging-drop (E) culture medium from ME180 control and LATS1/2 knockdown cells. Each bar represents the mean ± SEM of five independent experimental results. Bars with different letters are significantly different from each other [CTRL vs. siLATS1/2 in (D), P = 0.0017; CTRL vs. siLATS1/2 in (E), P = 0.0045].

Data information: Data in (B), (D), and (E) were analyzed for significance with unpaired t-test in GraphPad Prism 5 with Welch’s correction.