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. 2015 Sep 28;7(11):1426–1449. doi: 10.15252/emmm.201404976

Figure EV5. Knockdown of EGFR suppressed YAP-induced cell growth in cancerous cervical cells.

Figure EV5

  1. Western blot analysis showed that siRNAs of EGFR successfully knocked down EGFR in ME180 cells.
  2. Knockdown of EGFR blocked YAP-induced proliferation of ME180 cells. Each bar represents the mean ± SEM of five independent experimental results. Bars with different letters are significantly different from each other (CTRL-MXIV vs. siEGFR-MXIV, P = 0.0167; CTRL-YAP vs. siEGFR-YAP, P < 0.0001; CTRL-YAPS127A vs. siEGFR-YAPS127A, P < 0.0001).
  3. EGFR knockdown or verteporfin treatment dramatically decreased YAP-induced AREG secretion. Each bar represents the mean ± SEM of five independent experimental results. Bars with different letters are significantly different from each other (CTRL-MXIV vs. siEGFR-MXIV, P = 0.0006; CTRL-MXIV vs. VTPF-MXIV, P < 0.0001; CTRL-YAP vs. siEGFR-YAP, P = 0.0007; CTRL-YAP vs. VTPF-YAP, P < 0.0001; CTRL-YAPS127A vs. siEGFR-YAPS127A, P < 0.0001; CTRL-YAPS127A vs. VTPF-YAPS127A, P < 0.0001).

Data information: Data in (B) and (C) were analyzed for significance using two-way ANOVA in GraphPad Prism 5.