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. 2015 Oct 16;7(11):1465–1479. doi: 10.15252/emmm.201505344

Figure 2. Proteomic characterization of the human blastocoel fluid.

Figure 2

  • A About 182 proteins identified from the blastocoel fluid had corresponding mRNA transcripts detected in embryos. The total number of proteins identified according to type of sample preparation method used is shown (red, urea; blue, MonoPrep; purple, shared; green, total). Full and barred sections refer to proteins confirmed and unconfirmed by gene expression of whole human blastocysts using microarrays, respectively.
  • B The subcortical maternal complex (SCMC) is shown as an example of embryo-specific proteins identified. Complex subunits identified with mass spectrometry (MS) are shown in red, and protein interactions were derived from STRING (Jensen et al, 2009). Multiple lines indicate the existence of different evidences in the STRING database supporting the interaction.
  • C, D Gene Ontology (GO) classification indicating subcellular localization (C) and function (D) of the 182 blastocoel proteins identified by MS and validated with gene expression analysis. In (C), proteins localized in the extracellular space were highlighted as potentially involved in autocrine or paracrine signaling. In (D), groups with functions associated with embryo development and implantation processes were highlighted. GO annotations were obtained using Protein Center software (Thermo Fisher, USA). Note: Most proteins have multiple annotations.