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. 2015 Oct 16;7(11):1465–1479. doi: 10.15252/emmm.201505344

Figure 4. Blastocentesis is a safe procedure for the embryo, and it improves survival and blastocoel re-expansion following vitrification.

Figure 4

  1. Time-lapse photography of a human blastocyst collapsed using microsuction prior to cryopreservation or culture (left) and subsequent re-expansion to normal morphology (right). Scale bars: 50 μm.
  2. Re-expansion rates of human blastocysts treated with microsuction prior to incubation for 5 and 24 h.
  3. Embryo survival (left) and full re-expansion (right) rates in human blastocysts treated with microsuction or untreated prior to vitrification/warming and culture for 5 h. P-values calculated using two-tailed Fisher’s exact test (C.I. 95%).