Figure EV3. Validation of embryonic origin of selected MS-identified proteins using PCR.

Total mRNA was isolated from 9 whole blastocysts. Four target transcripts (granulins, GRN; oocyte-expressed protein, OOEP; NACHT, LRR, and PYD domains-containing proteins 5, NLRP 5; and NACHT, LRR, and PYD domains-containing proteins 7, NLRP 7) were amplified using nested PCR and custom-built primer sets. Expected bands were excised from the gel and subjected to Sanger sequencing to confirm the presence of the correct transcripts.